RNA interference : methods for plants and animals /

• Format: eBook
• Language: English
• Published: Wallingford, Oxfordshire, UK : CABI, 2009.
• Series: Principles and protocols.
• Subjects: Gene silencing.; RNA, Small Interfering > physiology.; RNA Interference.; Small interfering RNA.; Plant Breeding and Genetics.; Animal Genetics and Breeding, (New March 2000); Genetic Engineering, Gene Transfer and Transgenics, (New June 2002); Molecular Biology and Molecular Genetics, (Discontinued March 2000, Reinstated and Revised June 2002); Animal breeding.; Animal genetic resources.; Chromatin.; Gene expression.; Genes.; Genetic engineering.; Genomes.; Phenotypes.; Plant breeding.; Regulatory genes.; RNA.; RNA interference.; Electronic books.
• Online Access: View fulltext via EzAccess

The discovery that small RNA molecules act through RNA interference (RNAi) pathways as potent regulators of chromatin structure, messenger RNA (mRNA) abundance and translation, has revolutionized our understanding of gene regulation. These small RNA silencing systems have also been harnessed as powe...

Table of Contents:

• Hairpin RNAi in plants / Louisa Matthew
• Virus-induced gene silencing (VIGS) to study gene function in plants / Xiaohong Zhu and Savithramma P. Dinesh-Kumar
• The use of artificial microRNAs in targeted gene silencing / John Paul Alvarez and Yuval Eshed
• RNAi in Caenorhabditis elegans / Nicholas M. Johnson, Julie-Anne Fritz and Carolyn A. Behm
• RNAi in Drosophila / Steven Whyard
• RNAi in fish and crustaceans / Mario Pablo Estrada, Juana María Lugo and Yamila Carpio
• RNAi in chicken embryos / Nick J. Van Hateren, Raman M. Das and Stuart A. Wilson
• RNAi in mammalian cells / Yan Zeng
• siRNA and shRNA design / Debra J. Taxman.