Application of Selected Reaction Monitoring to Highly Multiplexed Targeted Quantitative Proteomics A Replacement for Western Blot Analysis /

• Main Authors: Kinter, Michael. (Author), Kinter, Caroline S. (Author)
• Corporate Author: SpringerLink (Online service)
• Format: Electronic
• Language: English
• Published: New York, NY : Springer New York : Imprint: Springer, 2013.
• Series: SpringerBriefs in Systems Biology,
• Subjects: Medicine.; Mass spectrometry.; Biochemistry.; Proteomics.; Biomedicine.; Biomedicine general.; Mass Spectrometry.; Biochemistry, general.
• Online Access: https://ezaccess.library.uitm.edu.my/login?url=http://dx.doi.org/10.1007/978-1-4614-8666-4

� A key experiment in biomedical research is monitoring the expression of different proteins in order to detect changes that occur in biological systems under different experimental conditions. �The method that is most widely used is�the Western blot analysis.� While Western blot is a workhorse in l...

Table of Contents:

• The use of mass spectrometry for highly selective detection
• overview of how the selected reaction monitoring experiment works
• Designing a selected reaction monitoring method for a protein
• Example analyses include: sample processing, sample analysis, data processing
• Future Directions.